Larry Daley gelooft, ondanks zijn tot op heden ongelukkige leven, dat er iets groots voor hem staat te gebeuren. Dat blijkt wel als hij aangenomen wordt als nachtwaker bij het "Museum of Natural History". Tijdens zijn diensten beginnen er vreemde dingen te gebeuren. Gladiatoren en cowboys voeren een nachtelijke strijd terwijl een Neanderthaler zijn eigen display verbrandt, en de T-rex iedereen laat zien waarom hij het meest gevreesde roofdier is uit de geschiedenis.
Nee, helaas is Night at the Museum nog niet beschikbaar op Netflix in Nederland.
| Editing | Don Zimmerman | Editor |
| Art | Claude Paré | Production Design |
| Art | Bridget McGuire | Art Direction |
| Art | Grant Van Der Slagt | Art Direction |
| Art | Michele Laliberte | Art Direction |
| Writing | Robert Ben Garant | Screenplay |
| Production | Michael Barnathan | Producer |
| Art | Lin MacDonald | Set Decoration |
| Production | Shawn Levy | Producer |
| Directing | Shawn Levy | Director |
| Writing | Thomas Lennon | Screenplay |
| Production | Ilene Starger | Casting |
| Production | Chris Columbus | Producer |
| Sound | Alan Silvestri | Original Music Composer |
| Art | Michael Diner | Art Direction |
| Camera | Guillermo Navarro | Director of Photography |
| Sound | Stefán Karl Stefánsson | ADR & Dubbing |
| Writing | Milan Trenc | Book |
| Visual Effects | Julie D'Antoni | Visual Effects Producer |
| Crew | Clint Carleton | Stunts |
| Crew | Lauro David Chartrand-DelValle | Stunts |
| Crew | Kimberly Chiang | Stunts |
| Sound | Armen Ksajikian | Musician |
| Sound | Craig Ricci Shaynak | ADR & Dubbing |
| Crew | J.J. Makaro | Stunt Coordinator |
| Art | Helen Jarvis | Supervising Art Director |
| Editing | Dean Zimmerman | Associate Editor |
| Art | Chris Beach | Set Designer |
| Crew | Chad Sayn | Stunt Double |
| Crew | Yves Cameron | Stunts |
| Crew | Jeffrey C. Robinson | Fight Choreographer |
| Crew | Rob 'Sluggo' Boyce | Stunts |
| Crew | Loyd Bateman | Stunts |
| Crew | Mike Mitchell | Stunt Double |
| Crew | Paul Rutledge | Stunts |
| Crew | Jon Kralt | Stunts |
| Crew | Dave Hospes | Stunts |
| Crew | Dean Choe | Stunts |
| Crew | Kit Mallet | Stunts |
| Crew | Mike Desabrais | Stunts |
| Art | John Dale | Construction Foreman |
| Sound | Warren Hendriks | First Assistant Sound Editor |
| Sound | Tim Gomillion | Sound Recordist |
| Crew | William H. Orr | Special Effects Coordinator |
| Crew | Gloria Davies | Unit Publicist |
| Sound | Matt Patterson | Sound Recordist |
| Sound | Craig Henighan | Sound Designer |
| Sound | R.J. Kizer | ADR Supervisor |
| Sound | Donald Sylvester | Foley Editor |
| Camera | Neil McBride | Dolly Grip |
| Costume & Make-Up | Karen Patch | Wardrobe Designer |
| Costume & Make-Up | Blanche Boileau | Costume Supervisor |
| Production | Neil Robertson | Location Manager |
| Art | Jan Kobylka | Construction Coordinator |
| Crew | Rob Vreugde | Transportation Coordinator |
| Sound | John Murray | Foley Supervisor |
| Costume & Make-Up | Victoria Down | Makeup Artist |
| Sound | Dennis Rogers | Sound Recordist |
| Sound | Helen Luttrell | Dialogue Editor |
| Sound | Dave Kulczycki | Sound Effects Editor |
| Sound | Skip Longfellow | Assistant Sound Editor |
| Costume & Make-Up | Sherry Linder-Gygli | Hairstylist |
| Sound | Susan Dawes | Dialogue Editor |
| Camera | James Salberg | Dolly Grip |
| Sound | Mildred Iatrou | ADR Editor |
| Sound | Terry Wilson | Supervising Music Editor |
| Sound | David Lucarelli | ADR Recordist |
| Sound | John T. Cucci | Foley Artist |
| Editing | Gary Burritt | Negative Cutter |
| Sound | Dan O'Connell | Foley Artist |
| Sound | Charleen Richards-Steeves | ADR Mixer |
| Editing | Chris Regan | Color Timer |
| Sound | James Ashwill | Foley Mixer |
| Camera | Larry Blanford | Second Unit Director of Photography |
| Directing | Diane Durant | Script Supervisor |
| Costume & Make-Up | Renée April | Costume Design |
| Directing | Daniel Mansfield | Second Second Assistant Director |
| Sound | Michael Hibberson | Boom Operator |
| Visual Effects | Jim Rygiel | Visual Effects Supervisor |
| Directing | Rhonda Taylor | Third Assistant Director |
| Sound | Rob Young | Production Sound Mixer |
| Sound | Craig Henighan | Supervising Sound Editor |
| Production | Ellen Somers | Associate Producer |
| Production | Anne M. Simonet | Unit Production Manager |
| Directing | Josh McLaglen | First Assistant Director |
| Production | Josh McLaglen | Co-Producer |
| Production | Ira Shuman | Executive Producer |
| Sound | John A. Larsen | Supervising Sound Editor |
| Production | James Forsyth | Extras Casting |
| Production | Mark Radcliffe | Executive Producer |
| Production | Thomas M. Hammel | Unit Production Manager |
| Camera | Scott Cozens | Camera Loader |
| Crew | Blaise Corrigan | Stunt Driver |
| Art | Glenn Bydwell | Set Designer |
| Art | Ellen Freund | Property Master |
| Directing | Marty Eli Schwartz | First Assistant Director |
| Camera | David Crone | "A" Camera Operator |
| Directing | Brian Smrz | Second Unit Director |
| Art | Peter Bodnarus | Set Designer |
| Directing | Misha Bukowski | Second Assistant Director |
| Production | Ira Shuman | Unit Production Manager |
| Art | Doug Girling | Set Designer |
| Camera | Glen A. Dickson | "B" Camera Operator |
| Production | Thomas M. Hammel | Executive Producer |
| Art | Ray Lai | Graphic Designer |
| Art | Loree Cameron | Graphic Designer |
| Production | Zoe E. Rotter | Casting Associate |
| Production | Valerie Lindquist | Casting Assistant |
| Visual Effects | Dan DeLeeuw | Visual Effects Supervisor |
