Andy is een conservatieve, verlegen man en nog steeds maagd. Als zijn vrienden hier achter komen, doen ze er alles aan om hem aan de vrouw te krijgen. Andy speelt mee maar komt in de ene na de andere rampzalige situatie terecht. Totdat... hij een relatie krijgt met Trish, een vrouw met drie kinderen. 'Eindelijk', denken zijn vrienden, 'nu gaat het gebeuren!' Maar nee, Andy en Trish houden hun relatie voorlopig strikt platonisch...
Nee, helaas is The 40 Year Old Virgin nog niet beschikbaar op Netflix in Nederland.
| Writing | Judd Apatow | Screenplay |
| Directing | Judd Apatow | Director |
| Writing | Steve Carell | Screenplay |
| Production | Judd Apatow | Producer |
| Sound | Lyle Workman | Original Music Composer |
| Editing | Brent White | Editor |
| Costume & Make-Up | Gary Archer | Prosthetics |
| Production | Andrew J. Cohen | Associate Producer |
| Production | Marla Garlin | Casting |
| Art | K.C. Fox | Set Decoration |
| Production | Clayton Townsend | Producer |
| Sound | George H. Anderson | Supervising Sound Editor |
| Production | Steve Carell | Executive Producer |
| Production | Shauna Robertson | Producer |
| Camera | Jack N. Green | Director of Photography |
| Art | Jackson De Govia | Production Design |
| Production | Jon Poll | Executive Producer |
| Production | Seth Rogen | Co-Producer |
| Art | Tom Reta | Art Direction |
| Production | Allison Jones | Casting |
| Sound | Cindy Marty | Sound Effects Editor |
| Costume & Make-Up | Debra McGuire | Costume Design |
| Crew | Tim Gilbert | Stunts |
| Crew | Zachary Woodlee | Associate Choreographer |
| Crew | Debbie Evans | Stunts |
| Crew | Buddy Joe Hooker | Stunt Coordinator |
| Directing | Peter Dress | Second Second Assistant Director |
| Crew | Anne Fletcher | Choreographer |
| Crew | Eddy Donno | Stunts |
| Crew | Tony Donno | Stunts |
| Crew | Erik Stabenau | Stunts |
| Crew | William H. Burton Jr. | Stunts |
| Crew | Gayle Hooker | Stunts |
| Crew | Greg Harris | Stunts |
| Crew | Shawn Kautz | Stunts |
| Crew | Jayme Jensen | Stunts |
| Crew | Chad Randall | Stunts |
| Crew | Ralph W. Odum | Stunts |
| Crew | Scott Wilder | Stunts |
| Crew | Jimmy N. Roberts | Stunts |
| Directing | Rusty Mahmood | Second Assistant Director |
| Directing | Michele Panelli-Venetis | First Assistant Director |
| Crew | Richard Stutsman | Special Effects |
| Crew | William H. Schirmer | Special Effects |
